The Biological effects of uritica dioica extraction on some pathogenic Bacteria

Abstract

The Urtica dioica is a flowering plant use in folk medicine, and as a food source. Nettles grow all over 
the world in mild to temperate climates. The study aimed to determine the antibacterial activity of alcoholic watery 
extracts of dry leaves of Urtica dioica on some pathogenic microorganisms (E.coli, Staphylococcus. aureus). The 
Plants were collected in the early time of January from Baghdad. Deride leaves of Uritica were extracted using 
co-solvent (Water and ethanol) extraction way by Soxhelt apparatus for crude extraction, and use rotary evaporator 
to obtain power extract. The series dilution was prepared 50%, 25, 12.5w/v that use on Mueller-Hinton agar plates 
against the St.aureus, E.coli by using well diffusion method then compared with antimicrobial activity of many 
antibiotics against the same isolates using Kirby-Bauer method. The crude extract of U. dioica was showed a 
significant antibacterial effect against Staph.aureus : at the concentration 50% the inhibition zone was 20 mm , 
followed by 25% (14mm) , and12.5%(10mm), and E.coli : at the concentration 50% the inhibition zone was18mm 
, followed by 25%(12mm) ,and 12.5(8mm) respectively. Based on the obtained results indicated the U. dioica 
plant is very useful used as antibacterial agent in treating bacterial infections.